Developmental Neurobiologist @UZH_Science | Microscopy Enthusiast | Science communication | Bluesky: @axonsalex.bsky.social Youtube: @AlexNeuroHealth
Axonal growth cone
Joined August 2020
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Can small extracellular vesicles guide growing axons?
Our new preprint provides evidence that small extracellular vesicle (sEV) secretion regulates axon guidance at a key ventral midline choice point during spinal cord development.
biorxiv.org/content/10.64898…
#neuroscience #EV
The tissue shown here is an ex vivo culture of intact spinal cord from a🐣embryo, allowing us to observe these processes in a controlled yet physiologically relevant setting.What looks like a simple crossing event emerges from complex and tightly regulated cellular processes.
During development, these neurons extend long projections that grow toward the midline, cross it, and then turn to grow along the length of the spinal cord. This coordinated behavior is essential for establishing connectivity between the two sides of the nervous system.
These are dI1 neurons, a well-studied class of spinal interneurons that have been used for more than 30 years to uncover fundamental mechanisms of axon guidance and neural circuit formation.
Wiring Both Sides of the Nervous System 🔬
#FluorescenceFriday #Neuroscience #DevelopmentalBiology #Microscopy #LifeSciences
This video shows axons crossing the midline of the developing spinal cord.
A Sticky Dance Between a Cell and a Neuron. #neuroscience #science #mysterious #HiddenWorld #cell #FluorescenceFriday
➡️A Schwann cell precursor interacting with sensory axons 🔬🧫
This is how actin shapes the neuronal growth cone 🔬
When a neuron grows, it doesn’t just extend blindly. It actively moves, explores its surroundings, and makes decisions along the way.
#Neuroscience
#ScienceCommunication
#Microscopy
#brain
#Science #LifeSciences #neuron
more videos on my new youtube channel: youtube.com/@AlexNeuroHealth
This Is What Drives Axon Growth. Axon growth is powered by the actin cytoskeleton at the growth cone.
Here, filamentous actin dynamics drive fast, directed axon extension in real time. #Fluorescencefriday #science #neuroscience
Here’s an embryonic axonal growth cone labeled with PK Mem dye (red) and tubulin (blue), imaged every 2 seconds. 🤩
Thrilled to collaborate with @ZhixingChen2 Lab @PKU1898!
We tested new PK Mem dyes—gentle, photostable tools to label the plasma membrane in live neurons, making it easier to track growth cone motility & axonal transport. #liveimaging #neuroscience
tiny.cc/3qhp001
This time-lapse captures 17h of axonal growth from a chick dorsal root ganglion explant, seen through the actin cytoskeleton using live imaging. I just submitted this video to the Nikon Small World in Motion competition. Today is the last day to upload yours!😉 #neuroscience
Alexandre Dumoulin @axonsalex.bsky.social retweeted
Come and join the Williams lab @OfficialUoM @FBMH_UoM @The_MRC We are using single-cell Multiomics and in vivo CRISPR approaches to understand the molecular mechanisms underlying lineage segregation from the neural plate border 🐣jobs.manchester.ac.uk/Job/Jo…
Alexandre Dumoulin @axonsalex.bsky.social retweeted
I’m very excited to finally present something we’ve been working on for the past couple of years – a spatial proteome map of primary cilia, released in @ProteinAtlas today. Amazing teamwork led by @jn_hansen
Check out our preprint doi.org/10.1101/2024.10.20.6…
Alexandre Dumoulin @axonsalex.bsky.social retweeted
The punishment for science misconduct is …. nothing. All these men are still running labs / paid professors.
List of high-level Alzheimer's researchers accused of credible fraud:🧵
#1 - Marc Tessier-Lavigne
Genentech pointed out problems in his 2009 Nature paper in 2012. After an expose by @tab_delete, he had to resign from Stanford Presidency, but can still run lab and do research.
Alexandre Dumoulin @axonsalex.bsky.social retweeted
Baby you’re a fiiiiirework!!!
Explanted Xenopus neural crest cell, microtubules (green) and actin (magenta). Imaging in frogs rocks- this is done room temp, no incubation, no problem. 🐸🔬✨
By Micaela Lasser, Helen Willsey @goodfrognosis Lab
@zeiss_micro LSM980 fast airyscan
Thanks Luc!
It is again the Pkmem 555 probe (magenta) and a mStayGold fusion protein (green). I don't have to correct my recordings for breaching anymore! there is no apparent bleaching to be seen...🤯🤯🤯
Time-lapse recording of floorplate cytonemes (green) interacting with axons (magenta). 1 image taken every second for 10 min (played at 30 fps). Cytonemes love interacting with axons! 💚#FluorescenceFriday
Alexandre Dumoulin @axonsalex.bsky.social retweeted
Retinoic acid, an essential component of the roof plate organizer, promotes the spatiotemporal segregation of dorsal neural fates
Read this #OpenAccess Research Article by @DinaRekler, Shai Ofek, Sarah Kagan, Gilgi Friedlander & @ChayaKalcheim @HebrewU:
journals.biologists.com/dev/…
ALT Figure 1: Assignment of transcriptomes to cell identities. (A) Experimental design for isolating single dorsal NT cells. (B,C) NTs electroporated at E2.5 and microdissected at E4, showing similar Msx1-Citrine labeling between control and RARα403 treatments. (D) UMAP of control and treated samples, colored by cluster with cell count indicated