M.D. | Physicist | Dr. rer. nat. | Investigating Tissue and Neurodegeneration by #CryoET, PostDoc @uniGoettingen, previous @MPI_Biochem
Munich, Germany
Joined July 2009
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Happy to share our latest review @CellCellPress with @ruferbus and Wolfgang Baumeister about high resolution imaging methods (#cryoET #SuperResolution) to investigate neurotoxic protein aggregates in #neurodegeneration. ➡️doi.org/10.1016/j.tcb.2020.0…
Felix Bäuerlein retweeted
Get ready for #SPACEtomo 🚀🌌 - fully automated parallel tomography on #cryoFIB lamellae. 🤖🔬❄️🦠 Another amazing development by @EisFabian 🙂👍
biorxiv.org/content/10.1101/…
We developed "DeepFIBing" - a new strategy to create #cryoFIB-lamellae for #cryoET in any depth of very large cells - this also works for tissue. If you want to know more, have a look into our publication in CELL with @jentoft_ida and @SchuhLab. #teamtomo bit.ly/3u3Azp6
Advantages of DeepFIBing:
- A simple method, which allows to create lamellae in any depth inside large specimens.
- Ice crystal contamination becomes irrelevant: a new contamination-free surface is created.
- Does not require high-pressure freezing or lift-out equipment.
Felix Bäuerlein retweeted
Now online! Mammalian oocytes store proteins for the early embryo on cytoplasmic lattices dlvr.it/SyJTct
Felix Bäuerlein retweeted
What is the optimal thickness of a Cryo-FIB lamellae? We quantify the tradeoff between specimen thickness and FIB damage by subtomogram averaging. There is no need to go ultra thin. Work by M Tuijtel, B Turonova @jpkreysing @sonjawelsch @HummerLab now online. @MPIbp #teamtomo
Thinner is not always better: Optimising cryo lamellae for subtomogram averaging biorxiv.org/cgi/content/shor… #bioRxiv
Felix Bäuerlein retweeted
We're hiring!
We're looking for an Image Analysis Specialist to develop methods for cryo-ET data mining 🖥️
The position can become permanent upon successful evaluation.
Come join us @uniGoettingen @yourUMG!
#teamtomo #cryoEM
umg.eu/karriere/stellenangeb…
Felix Bäuerlein retweeted
What an honor to receive a Consolidator grant from @ERC_Research !! We are excited to dive into nervous tissues using #cryoET in our #cryoNERVE project 🤩
Thanks to everyone who made this possible!
It was quite a ride to pick the LS1 @ERC_Research #ERCCoG 2022 grantees from such a great group of people so HUGE congratulations to: @ruferbus , @DoraTang5 , @Hashemyaser4 , @LaineElodie , @LandauMeytal , @TzviyaZBM , @YiliangDing and Stefano Pagliara (@LSI_Exeter) !!!! 🥳
Felix Bäuerlein retweeted
Congratulations to Wolfgang Baumeister for receiving the Alexander Hollaender Award in Biophysics 2022.
➡️nasonline.org/news-and-multi…
➡️nasonline.org/programs/award…
#CryoEM #NASaward
@theNASciences
Do you wonder about the future potential of #cryoET?
Will #AlphaFold replace experimental methods in structural biology or complement them?
In our new review with Wolfgang Baumeister we discuss these questions in the Special Issue “AlphaFold” @JMolBiol.
doi.org/10.1016/j.jmb.2021.1…
Felix Bäuerlein retweeted
We are looking for a new colleague working with us in lovely Groningen on protein aggregation and neurodegenerative diseases bit.ly/3kY2znB
Felix Bäuerlein retweeted
25 years ago I was measuring whales stranded dead along Sardinian coast, or releasing back to sea those found alive. Now our cryo-EM paper on the cetacean morbillivirus nucleoprotein-RNA complex is out on @JStructBiol sciencedirect.com/science/ar…
Felix Bäuerlein retweeted
We have updated our joint preprint with @ruferbus on #lysosome defects caused by protein aggregation, and added new data on the mechanism:
Amyloid-like aggregates cause lysosomal defects in neurons via gain-of-function toxicity biorxiv.org/content/10.1101/…
Felix Bäuerlein retweeted
Been working with @phospho animation on amyloid aggregation. 1st effort is pretty stunning, I think! This is an in vitro, wild-type hIAPP fibril from @R_U_Gallardo @RadfordLab lovely paper in @NatureSMB @Astbury_BSL @AstburyCentre #LoveTheWigglyBits
rdcu.be/b7bGc
Felix Bäuerlein retweeted
In practice, is #cryoET limited to cells in culture? In this manuscript we show an easy way to image native tissues with molecular detail. 1/5
biorxiv.org/content/10.1101/…
Are you interested in #cryo-ET of native tissues?
In our new pre-print we propose an easy applicable approach using plunge freezing and #cryoFIB to investigate vitrified tissue by #cryoET. With Jose Pastor-Pareja & @ruferbus. #cryoET_histology #cryoEM
doi.org/10.1101/2021.04.14.4…
1/4
#cryoFIB lamellae come often with disturbing preparation artifacts. We developed an algorithm, that computationally removes these and facilitates the interpretation of the rich context of these lamella overviews: „Lamella in silico Clearing“ (LisC)
4/4
Many physiological & pathological processes depend on the complex association of cells and extracellular structures in the tissue context which cannot be recapitulated in culture. Visualized here by #cryoET: a peripheral nerve with axons, perineurial glia & basement membrane
3/4
We demonstrate the applicability of this approach on nervous tissue, muscle tissue and epithelial tissue with molecular resolution. #cryoET_histology
2/4
ALT Cryo-ET tissue gallery of nervous, muscle & epithelial tissue. (Cryo-ET Histology)
Felix Bäuerlein retweeted
If you want to literally play with a microscope, try our new LEGO based one: biorxiv.org/content/10.1101/…. Plans and multi-language guides to teach kids are on GitHub: github.com/tobetz/LegoMicros…. Sub-micron resolution thanks to a smartphone lens. Please RT, so that it reaches the kids!