Roman Zubarev’s group at Karolinska Institutet focuses on Molecular Biometry using mass spectrometry for studying various processes of biomedical importance.
Stockholm, Sweden
Joined December 2019
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Noise isn’t always bad! Sometimes it helps us see the unseen. 🚀 Our new preprint shows how stochastic resonance boosts single-cell proteomics signals. 👉 biorxiv.org/content/10.1101/…
#Proteomics #SingleCell #MassSpec #Preprint
For decades, scientists believed that stable isotopes in our tissues reflected our diet with a small correction. Our new preprint shows it’s more complex: isotopes actively shape biology and converge in surprising ways.
Read the preprint 👉 [biorxiv.org/content/10.1101/…]
#isotopes #
🔬 Exciting News! 🎉 Our manuscript "Ultralight Ultrafast Enzymes" has been accepted by Angewandte Chemie! 📚 onlinelibrary.wiley.com/doi/…
🧪 Our hypothesis? Diminished conformational and vibrational entropy leading to accelerated kinetics.
🔬 Potential applications? Ultralight enzymes for scenarios requiring extreme reaction rates! ⚙️
Grateful for the collaboration and support from our amazing team! 🤝👩🔬👨🔬
#ScientificBreakthrough #Research #AngewandteChemie #IsotopeDepletion #EnzymeEngineering #UltralightUltrafastEnzymes
RZLab retweeted
Peptide sequencing based on host–guest interaction-assisted nanopore sensing - Nature Methods nature.com/articles/s41592-0…
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#proteomics #prot-paper
New paper published. Hopefully our results will lead to developing novel biomarkers and therapeutic interventions against traumatic brain injury in the long run.
Our paper on identification of biomarkers and therapeutic targets in traumatic brain injury (TBI) is now published. Here, we use PISA to dissect the early molecular events and proteome solubility changes following a dynamic injury to neurons.
mdpi.com/1420-3049/28/19/676…
Check out our new work on PISA-REX for analysis of proteomes in the three dimensions of redox state, solubility and expression for target identification.
Finally after a year of data analysis, fine-tuning and writing, one of my most important papers is online. It is about a tool for 3D analysis of proteomes in target deconvolution (1).
biorxiv.org/content/10.1101/…
RZLab retweeted
This toy example may change how you look at tSNE & UMAP.
They find clusters in the most uniform data possible.
RZLab retweeted
Our new collaborative study shows that NCF1-dependent production of ROS protects against lupus by regulating plasmacytoid dendritic cell (pDC) development and functions. ROS deficiency enhances pDC generation through the AKT/mTOR pathway.
insight.jci.org/articles/vie…
RZLab retweeted
DOPA is just as good (or even better!) than Vitamin C - when it comes to protecting tissues from free radicals. 🛡️🔥 Proud of this largely experimental (!) work of our lab. 🎉🎉🎉 Thanks to amazing collaborators @RedoxDKFZ @RZLabKI @prisnerlab and others!
1st author Cluster Young Scientist Markus Kurth uncovers collagen, the main structural protein of all connective tissues, as a redox-active material & establishes DOPA residues as redox-active side chains of collagens 👉 biorxiv.org/content/10.1101/… @biorxivpreprint #preprint
New work from our lab at @karolinskainst uncovers massive solubility changes of neuronal proteins upon simulation of traumatic brain injury. #TBI #neuroscience #proteomics #TeamMassSpec
Excited to share our work, where we combine a novel apparatus with state-of-the-art solubility proteomics and simulate traumatic brain injury, to uncover mechanisms of neuron damage, for ultimate identification of diagnostic and therapeutic biomarkers.
biorxiv.org/content/10.1101/…
RZLab retweeted
Happy Sunday 🙂
Please check out our new protocol - "Proteome Integral Solubility Alteration (PISA) Assay in Mammalian Cells for Deep, High-Confidence, and High-Throughput Target Deconvolution".
bio-protocol.org/e4556
@RZLabKI @XuepeiZhang
#proteomics #spectrometry #cells
RZLab retweeted
Finally, all pieces have been put together! The #FTMS Booster is now installed in the @RZLabKI. Thank you @Spectroswiss for all the help and also for a great talk!
Individual amino acid isotopic ratio measurement and peptide sequencing using only 1 μg of sample, all in one!
Fourier Transform Isotopic Ratio Mass Spectrometry enables precise measurements of isotopic composition of the elements C, H, N, and O in individual amino acids using only 1 μg of protein sample.
@RZLabKI @hasangharibi @chernolab @AmirAta8
pubs.acs.org/doi/10.1021/acs…
RZLab retweeted
The Omnitrap – IMS platform has successfully been installed at KI! Thank you @Fasmatech_MS , MSvision and @RZLabKI for the great effort. Time to produce some exciting data!
In our new preprint, we identify the O-glycosylated substrates of GALNT1 using versatile proteomics tools. Arguably one of the most challenging PTMs to study. We close in on a motif too.
Mapping the GALNT1 substrate landscape with versatile proteomics tools biorxiv.org/cgi/content/shor… #bioRxiv
Do you know isoaspartate acts as a molecular clock in aging? We collaborated with Prof. @CharlotteTeuni1 and Dr. Guo to test the link between isoaspartate and Alzheimer's disease etiology on A&D journal doi.org/10.1002/alz.12735 @alzdemjournals @alzassociation @ISTAART
