Geneticist interested in all aspects of neural development, especially Slit/Robo and axon guidance, as well as translational applications.
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ALT The Slit2ΔTLS allele retains dorsal midline repulsion of DRGs axons. (A,F) Dorsal root ganglia (DRG) axons enter the spinal cord at the dorsal root entry zone (DREZ) and bifurcate to project anterior and posterior in the dorsolateral funiculus (DLF), avoiding the SLIT2-expressing roof plate (RP). A, anterior; D, dorsal; P, posterior; V, ventral. (B-E) βIII-tubulin labeled transverse sections of E10.5 spinal cords. Dorsal midline projection errors were seen in Slit2−/− (arrowhead), but not in Slit2ΔTLS/− or Tll1−/− embryos (empty arrowheads). Dorsal is up in all images. (B′-E′) Magnifications of the DREZ region (arrowhead). Brackets indicate DRG axons in the spinal cord. (G-J) DiI tracer label was placed in DRGs (asterisk) of whole E11.5 embryos. (G) Wild-type embryos had no axons projecting towards the roof plate (n=4 embryos). (H) Slit2−/− embryos had overshooting DRG axons that invaded the ventricular zone toward the midline (arrowhead; n=2 embryos). (I) Slit2ΔTLS/− embryos lack over
ALT Image of Misty Riddle
