Painful facts from our favorite technique #PhDLife #LabLife #ScienceFail Tag us to share your frustration
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1. Prepare the samples
2. Cast the gels
3. Clean bench and recast gels
4. Realize there's no buffer
5. Prepare buffer
6. Run the gel
7. Realize there's no Gly
8. Borrow Gly
9. Transfer
10. Block
11. 1ary antibody
12. Wash
13. 2ary antibody
14. Wash
15. Develop
16. Fail
17. Goto 1
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#WesternBlotting #WesternBlot
Western Blot Facts retweeted
Told someone once that I wanted a magic machine that turned a mouse into a western blot. They said they wanted to turn a blot into a mouse. Their idea was better
Western Blot Facts retweeted
Im spending 7-8 hours everyday in the lab yalls, my brain is busted if i see a single western blot i will start crying
Western Blot Facts retweeted
Hey @acmilan, it's okay I don't need my club to sign a €120m midfielder, I just need my bloody Western Blot to work tomorrow!
Western Blot Facts retweeted
I made an app to save time making figures and quantifying blots/gels.
sciugo.com
youtu.be/nDq93d59Pog
@PhDVoice @PostdocVoice @thoughtsofaphd @heysciencesam @PhDfriendSana
@WB_Facts @lab_shenanigans @OpenAcademics @AcademicChatter #phdlife #lablife #westernblot
Western Blot Facts retweeted
When you were about to block your western blot but the transfer didn’t work – at Cardiff, Wales
