@kptsilva

NCI

 Bethesda, MD
scholar.google.com/citations?user=swAQZr8AAAAJ&hl=en
Joined August 2012
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Check out our new work on identifying the genes involved in the formation and maintenance of gene amplifications in Staphylococcus aureus. @AnuKhareLab
bioRxiv Microbiology@biorxiv_micrbio12 Apr 2025
Genetic determinants of gene amplifications alter frequency and evolutionary trajectory of antibiotic resistance in Staphylococcus aureus biorxiv.org/content/10.1101/… #biorxiv_micrbio

Genetic determinants of gene amplifications alter frequency and evolutionary trajectory of antibi...

Gene amplifications are thought to be common in bacterial populations, providing a rapid reversible mode of adaptation to diverse stresses, including the acquisition of antibiotic resistance. We...

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Antibiotic resistance mediated by gene amplifications🧬 This review is available open-access to read @npjAMR! @kptsilva @AnuKhareLab (1/5) nature.com/articles/s44259-0…
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Publication alert 🚨: Our new review article on antibiotic resistance via distinct gene amplifications is out now in npj antimicrobials and resistance!!! Here is the link: nature.com/articles/s44259-0… #antibioticresistance #geneamplification
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Phenotypic heterogeneity is generated by passive mechanisms like noise in gene expression, right? Not always! Check out an ACTIVE mechanism for generating diversity in a clonal bacterial population: biorxiv.org/cgi/content/shor…
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Super cool research out in @NatureComms 🤩 Multicopy plasmids, often carrying antibiotic #resistance genes, can rapidly amplify genes, leading to transient antibiotic resistance 🦠💊 nature.com/articles/s41467-0…

Plasmid-mediated phenotypic noise leads to transient antibiotic resistance in bacteria

Nature Communications - In this work, authors combine computational models with single-cell and population-level data showing the variability in plasmid copy number within bacterial populations...

nature.com
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Fun fact: #Staphylococcus aureus divides using alternating division planes that are orthogonal to the previous one. But how? But why? @felixrl_, @braf_phd and collabs may have cracked this 50-y.o. mystery! #MRSA Our preprint: biorxiv.org/content/10.1101/…

Protein coopted from a phage restriction system dictates orthogonal cell division plane selection...

The spherical bacterium Staphylococcus aureus , a leading cause of nosocomial infections, undergoes binary fission by dividing in two alternating orthogonal planes, but the mechanism by which S....

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‘By 2050, not only will AMR perhaps be responsible for 10 million deaths annually it will cost the global economy over $100 trillion—this dwarfs the impact of COVID-19 and emphasize the urgent threat of AMR’ journals.plos.org/plosmedici…

Antimicrobial Resistance: Addressing a Global Threat to Humanity

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Important research out now in @NatureComms from @AnuKhareLab et al. 💫 Efflux pump SdrM underpins the evolution of #resistance to fourth-generation fluoroquinolone antibiotic delafloxacin in Staphylococcus aureus 🧩💊🦠 (🧵 1/5) nature.com/articles/s41467-0…
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Efflux pump gene amplifications bypass necessity of multiple target mutations for resistance against… disq.us/t/4gw9wzy
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We found that sdrM is required for the existence of the amplifications, and without sdrM, S. aureus always chooses to evolve multiple mutations in the DNA gyrase and topoisomerase. Therefore, the decision between the two alternate evolutionary trajectories is determined by sdrM.
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As a follow up to this paper, we are currently looking at possible factors that are initiating the amplifications. I would like to thank @AnuKhareLab for mentoring me through out this project. And, Ganesh Sundar for doing the very first evolution. #antimicrobialresistance#mrsa
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We also found out that the stability of these genomic amplifications can lead to cross resistance against Streptomycin. Mutations within sdrM seem to also emerge earlier than the emergence of any canonical mutations.
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it was generally thought that non-homologous recombination might not be present due to the absence of the genetic determinants seen in other bacteria.
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xitter.cf/AnuKhareLab/status/166… Happy to see out hard work finally getting published!
Anupama Khare@AnuKhareLab9 Jun 2023
Our work @NCIResearchCtr on the evolution of antibiotic resistance in #MRSA, led by postdoc @kptsilva is now out in @NatureComms! nature.com/articles/s41467-0…
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New preprint from our lab @NCIResearchCtr on #AMR in #MRSA #Saureus - we saw some surprising and cool results in this study led by postdoc @kptsilva ! biorxiv.org/content/10.1101/… A short🧵about our findings: 1/7

Efflux pump gene amplifications bypass necessity of multiple target mutations for resistance...

The rise of antimicrobial resistance has motivated the development of antibiotics that have multiple cellular targets, to theoretically reduce the frequency of resistance evolution, but adaptive...

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