@mbyaffe

Professor at MIT, Surgeon-Scientist. DNA damage signaling, protein kinases, precision cancer medicine, signaling in tumor microenvironment

 Boston, Massachusetts
Joined September 2009
  • Tweets137
  • Following126
  • Followers904
4Media
Scientists and universities: defend indirects as a concept but not current indirect rates. Don't think there's waste in those numbers? We (US science) spend $4.5 billion on journals. That is HALF of the proposed cuts in indirects, and eliminating that spending would make science better!
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Special thanks to Adam Palmer's lab, Matt Vander Heiden's lab, Omer Yilmaz's lab, and the folks at Genentech, all of whom were instrumental in this 5-FU story about RNA damage! Collaborative science is always the best!
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Thrilled to share our latest story on 5-FU inducing RNA damage during ribosome biogenesis - cell.com/cell-reports-medici…. The whole thing started when Karl and Jung-Kuei were trying to figure out hiow limiting dNTPS skewed DNA repair. Never thought it would be RNA and tge ribosome!

An RNA damage response network mediates the lethality of 5-FU in colorectal cancer

5-fluorouracil’s utility in cancer treatment has been attributed to inducing dNTP imbalance and DNA damage in cancer cells. Here, Chen et al. demonstrate that 5-FU’s efficacy results instead from RNA...

cell.com
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nature.com/articles/s41587-0… Thrilled to share this latest work led by Xiao-Kang Lun in Peng Yin's lab and Xueyang Yu in mine. Finally, single cell signaling can begin to rival single cell RNA-Seq, using CyTOF, phospho-antibodies and ACE amplification.

Signal amplification by cyclic extension enables high-sensitivity single-cell mass cytometry

Nature Biotechnology - Mass cytometry with signal amplification enables measurement of low-abundance proteins.

nature.com
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This is rich - one of the primary reasons scientists don't have a lot of time to think is that @nature and its ilk have promoted a culture where the primary goal of science is to produce bloated Nature papers that require lots of time and money, but little thought, to produce.
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Thrilled to share our characterization of the human tyrosine kinome. Try out the public website: kinase-library.mit.edu/home Wonderful collaboration with @CantleyLab @benturklab @LabYaffe. Many years of work led by Jared Johnson! nature.com/articles/s41586-0…
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This is a really important thread from Lior Pachter about the mathematical underpinnings of scRNAseq.
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threadreaderapp.com/thread/1…

Thread by @lpachter on Thread Reader App

@lpachter: The choice of whether to use Seurat or Scanpy for single-cell RNA-seq analysis typically comes down to a preference of R vs. Python. But do they produce the same results? In w/ @Josephmr...

threadreaderapp.com
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Excited to share our latest story - JNK and Erk MAPK signaling controls senescence from damaged replication forks, and at later times drives the SASP. Means MEK inhibitors with topoisomerase chemo likely to be a bad combo!
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authors.elsevier.com/c/1hRk6…
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This is truly brilliant… youtu.be/5uFwyPP5GOQ
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Fantastic motifs and signaling meeting at EMBL the last 2 days in honor of Toby Gibson.
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Links between translation and alternative polyadenylation in metastasis? The story is out today! A great collab between @genophoria, @AndreiGoga_SF and @FKHM labs! Shout out to co-authors @Gentremet, @JulianeScience, @lisacfishsci among others! nature.com/articles/s41556-0…
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Very grateful to our collaborators at Genentech, Adam Palmer's lab, @ac_palmer, Matt Vander Heiden's lab, @mvh_lab, and Omer Yilmaz's lab @ohyilmaz, and of course the Koch Institute! @kochinstitute
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In the bioRxiv paper, we find that 5-FU shows no synergy at the cellular level with the DNA damaging drugs oxaliplatin or irinotecan - the combination given for GI cancer Rx. Instead, ribosome and tRNA biogenesis is impaired, driving an apoptotic signal that does not require p53
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RNA Damage? Happy to share Jung-Kuei Chen and Karl Merrick and @LabYaffe preprint that reports the major mechanism of 5-FU action in clinically relevant cancer treatment is through damage to structured RNAs...biorxiv.org/content/10.1101/….

An RNA Damage Response Network Mediates the Lethality of 5-FU in Clinically Relevant Tumor Types

5-fluorouracil (5-FU) is a successful and broadly used anti-cancer therapeutic. A major mechanism of action of 5-FU is thought to be through thymidylate synthase (TYMS) inhibition resulting in dTTP...

biorxiv.org
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Michael Yaffe retweeted
Grant Stewart@GS_Lab_Bham29 Apr 2023
Great work from the @davidkcortez lab! doi.org/10.1126/science.add7…

RAD51 bypasses the CMG helicase to promote replication fork reversal

Fork reversal requires the RAD51 recombinase to overcome the CMG helicase, which remains bound to the stalled fork.

science.org
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If we didn’t have tenure scientists never would have risen up to discard our dysfunctional publishing system
Drug Monkey@drugmonkeyblog20 Apr 2023
What is your elevator pitch for academic tenure? That will resonate with your non-academic, working person neighbors?
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Congrats to Justine Stehn, Scott Floyd, and Yi Kong on what I hope will be the first of a series of papers showing alterations in protein translational control after DNA damage through stress signaling and 14-3-3. frontiersin.org/articles/10.…

Frontiers | MAPKAP Kinase-2 phosphorylation of PABPC1 controls its interaction with 14-3-3 proteins...

14-3-3 proteins play critical roles in controlling multiple aspects of the cellular response to stress and DNA damage including regulation of metabolism, cel...

frontiersin.org
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Michael Yaffe retweeted
CPRatUCPH@UCPH_CPR17 Apr 2023
Despite our scientific success @novonordiskfond will close CPR due to new funding strategy. #forskpol #DKforsk We applaud our visionary leader Jiri Lukas for creating a global flagship in protein research whose pioneering spirit will live on #CPRlegacy. How did he do this? 👇🧵
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