@GonenShane

Assistant Professor, University of California Irvine. All opinions my own #CryoEM ❄️ #ProteinDesign #Biochemistry #MembraneProteins #UCI ☀️

 Irvine, CA
cryoem.bio.uci.edu
Joined July 2018
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127Media
Very excited to start my new role as an Assistant Professor at the University of California Irvine. A very big thank you to my Postdoctoral advisor Yifan Cheng and the absolutely wonderful people in his lab!
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The Lawrence Lab website (lawrence-lab.org) is live and we are hiring! We combine high resolution structure🔬 and cell biology🧫🧪 approaches to define mechanisms of stress response signaling in health and disease. Live postings at technician and postdoc levels: PLZ RT!
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This 2D protein crystal
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Wow, massive congratulations David! @UWproteindesign
The Nobel Prize
@NobelPrize9 Oct 2024
BREAKING NEWS The Royal Swedish Academy of Sciences has decided to award the 2024 #NobelPrize in Chemistry with one half to David Baker “for computational protein design” and the other half jointly to Demis Hassabis and John M. Jumper “for protein structure prediction.”
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Is anyone else having issues with @LeicaMicro ? Our GP2 has had issues for a long time and requests over the last few months have gone nowhere
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Southern California Today
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Now all I see are enemies preventing Sonic from clipping #cryoEM grids
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I'm happy to finally present our #cryoEM work on Voltage-gated sodium channel 1.8 (Nav1.8) with a bound Tarantula peptide Protoxin-I!! Fabulous work from Bryan and Stephen 🥳🎉👌 biorxiv.org/content/10.1101/…

Structural basis of inhibition of human NaV1.8 by the tarantula venom peptide Protoxin-I

Voltage-gated sodium channels (NaVs) selectively permit diffusion of sodium ions across the cell membrane and, in excitable cells, are responsible for propagating action potentials. One of the nine...

biorxiv.org
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We also saw some large movements of the linker leading to VSDI and took advantage of graphene grids to get massive amounts of particles imaged from very little starting culture. 🥶🔬
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Full length human Nav yield in HEK cells is low and graphene grids ended up being a great alternative to drastic changes to the prep or construct.
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Expansion microscopy microscope
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We combined deep learning and comparative modeling to analyze some wicked disulfide rich ion-channel targeting toxins 🐚🐌 - awesome work from Stephen McCarthy in my lab👌 onlinelibrary.wiley.com/doi/…

δ‐Conotoxin Structure Prediction and Analysis through Large‐Scale Comparative and Deep Learning...

δ-Conotoxins are a diverse class of peptides that inhibit the inactivation of voltage-gated sodium channels – proteins implicated in many human diseases – but which remain poorly characterized....

advanced.onlinelibrary.wiley.com
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Is this grid ready for data collection?
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It's once again time to order grids. This time for our slated 2027 projects. 🤞we can get some by then
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Shane Gonen retweeted
Travis Wiles@bunsen_B14 Jun 2024
First preprint from the Wiles Lab! We used an approach we call "Phollow" to study phage replication and transmission by live imaging!
bioRxiv Microbiology@biorxiv_micrbio14 Jun 2024
Phollow: Visualizing Gut Bacteriophage Transmission within Microbial Communities and Living Animals biorxiv.org/cgi/content/shor… #biorxiv_micrbio
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When you give a talk at your institute and a neurosurgeon who attended and works on the same system as you asks to collaborate. @QuinaScience @UCIBioSci
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You think we need one more?
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this poor grid 😢
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Now everyone customize/share protein language models for their custom task/dataset via @GoogleColab 🤓 Paper: biorxiv.org/content/10.1101/… Colab: colab.research.google.com/dr… Credit: @LTEnjoy, Zhikai Li, @ChenchenHa42849, @BonnieSwt, Junjie Shan, @XibinBayesZhou, Dacheng Ma, @duguyuan
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