Views are my own.
Maryland, USA
Joined October 2015
- Tweets233
- Following75
- Followers103
- Likes390
Mike Kelly retweeted
The 10x Flex kit has worked well for fixed cell and fixed tissue samples. How does it do with FFPE samples? It took a bit of trial and error, but we have new data to show how it’s performing and wanted to share what we learned.
Mike Kelly retweeted
First run with the CytAssist-enabled Visum FFPE v2. Looking forward to the increased workflow flexibility this is going to make possible. @10xGenomics
I've decided to create a separate profile for work / science. Should have done this a while ago - since Twitter handles are now seemingly like orcidIDs and author names on publications... Please see @MCKellySCB
Mike Kelly retweeted
We've had a glut of failed 10x runs recently and it looks like we are not the ones. I've started a google doc to track down the problem lots/wells: docs.google.com/spreadsheets… please share! @LGMartelotto
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Mike Kelly retweeted
Protocol for nuclei isolation from fresh and frozen tissues using Salty-Ez10 buffer: compatible with snRNA-Seq and Multiome workflows from 10x Genomics protocols.io/view/protocol-f…
Mike Kelly retweeted
Interested in single-cell genomics but need help keeping up with a rapidly changing field? Check out our free #singlecellgenomicsday workshop, starting in a few minutes! Full agenda and livestream at satijalab.org/scgd, or follow along below:
youtube.com/watch?v=lX4d12nT…
Mike Kelly retweeted
Our free Single Cell Genomics Day livestream starts tomorrow at 10AM EST! You can ask questions during talks via twitter (include #singlecellgenomicsday) and we'll relay them to speakers, see you soon!
Mike Kelly retweeted
We are hiring! Looking for a new vole wrangler and experimental magician (behav., surgery, mol. bio, computation). Live in a beautiful place with awesome labmates. Relevant exp. a plus but training available. Pls retweet! @CUBoulderMCDB @CUBoulderBN
jobs.colorado.edu/jobs/JobDe…
We are recruiting a Scientist to support single cell with the intramural Childhood Cancer Data Initiative (CCDI) led by the NCI Pediatric Oncology Branch. A major goal of this Initiative is to establish tumor cell atlases for rare pediatric malignancies.
leidosbiomed.csod.com/ats/ca…
Mike Kelly retweeted
Interested in new methods for multimodal and integrative single-cell analysis? Check out our @cegs_ica virtual seminar series, where lead authors will present their work from new papers and preprints. Learn more and register at multimodalintegration.org/vi…
Mike Kelly retweeted
Four years' work, 178 billion sequencing reads, and nearly 5 million cells went into constructing atlases of gene expression and chromatin accessibility in human development, out now.
Mike Kelly retweeted
A seriously cool modification to #rnaseq that means you can see when individual promoters were active. The method is beautiful and simple.
Mike Kelly retweeted
We are excited to release:
* Our new preprint on weighted nearest neighbor analysis to define cell states based on multiple modalities in Seurat v4
* A multimodal CITE-seq dataset of human PBMC w/200k cells and 228 antibodies
biorxiv.org/content/10.1101/…
Data/code are available now!
Mike Kelly retweeted
We've been working on Seurat v4 for almost two years, and are excited to share it with you soon!
In a webinar w/@10xGenomics on 10/20, I'll introduce new methods to analyze multimodal single-cell data (i.e. from CITE-seq or the 10x multiome ATAC+RNA kit): 10xgenomics.com/event/integr…
Mike Kelly retweeted
My first thought when I saw this was - can you run this at home? And it turns out, you can!
biorxiv.org/content/10.1101/…
We are hiring for a data-focused Research Associate position in the NCI Single Cell Analysis Facility. Interested in the applying the latest and greatest single cell techniques to cutting edge cancer research at NIH Bethesda? More info here: bit.ly/3cgvZH6 Pls RT!
Mike Kelly retweeted
This is a great opportunity for someone interested in science communication and working to promote diversity in science. More info on the center here: multimodalintegration.org/
We are looking to hire a Director of Education and Outreach for @cegs_ica. If you'd like to help disseminate science and increase educational opportunities for students across NYC, please e-mail, DM, apply, or RT! More info at: nygenome-openhire.silkroad.c…
Mike Kelly retweeted
Excited to share our latest single cell multimodal assay. ASAP-seq pairs surface and intracellular protein detection with high quality chromatin accessibility on the @10xGenomics #scATACseq platform.
biorxiv.org/content/10.1101/…
