Views are my own.
Maryland, USA
Joined October 2015
- Tweets233
- Following75
- Followers103
- Likes390
Mike Kelly retweeted
The 10x Flex kit has worked well for fixed cell and fixed tissue samples. How does it do with FFPE samples? It took a bit of trial and error, but we have new data to show how it’s performing and wanted to share what we learned.
Mike Kelly retweeted
First run with the CytAssist-enabled Visum FFPE v2. Looking forward to the increased workflow flexibility this is going to make possible. @10xGenomics
Mike Kelly retweeted
We've had a glut of failed 10x runs recently and it looks like we are not the ones. I've started a google doc to track down the problem lots/wells: docs.google.com/spreadsheets… please share! @LGMartelotto
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Mike Kelly retweeted
Protocol for nuclei isolation from fresh and frozen tissues using Salty-Ez10 buffer: compatible with snRNA-Seq and Multiome workflows from 10x Genomics protocols.io/view/protocol-f…
Mike Kelly retweeted
Interested in single-cell genomics but need help keeping up with a rapidly changing field? Check out our free #singlecellgenomicsday workshop, starting in a few minutes! Full agenda and livestream at satijalab.org/scgd, or follow along below:
youtube.com/watch?v=lX4d12nT…
Mike Kelly retweeted
Our free Single Cell Genomics Day livestream starts tomorrow at 10AM EST! You can ask questions during talks via twitter (include #singlecellgenomicsday) and we'll relay them to speakers, see you soon!
Mike Kelly retweeted
We are hiring! Looking for a new vole wrangler and experimental magician (behav., surgery, mol. bio, computation). Live in a beautiful place with awesome labmates. Relevant exp. a plus but training available. Pls retweet! @CUBoulderMCDB @CUBoulderBN
jobs.colorado.edu/jobs/JobDe…
Mike Kelly retweeted
Interested in new methods for multimodal and integrative single-cell analysis? Check out our @cegs_ica virtual seminar series, where lead authors will present their work from new papers and preprints. Learn more and register at multimodalintegration.org/vi…
Mike Kelly retweeted
Four years' work, 178 billion sequencing reads, and nearly 5 million cells went into constructing atlases of gene expression and chromatin accessibility in human development, out now.
Mike Kelly retweeted
A seriously cool modification to #rnaseq that means you can see when individual promoters were active. The method is beautiful and simple.
Mike Kelly retweeted
We are excited to release:
* Our new preprint on weighted nearest neighbor analysis to define cell states based on multiple modalities in Seurat v4
* A multimodal CITE-seq dataset of human PBMC w/200k cells and 228 antibodies
biorxiv.org/content/10.1101/…
Data/code are available now!
Mike Kelly retweeted
We've been working on Seurat v4 for almost two years, and are excited to share it with you soon!
In a webinar w/@10xGenomics on 10/20, I'll introduce new methods to analyze multimodal single-cell data (i.e. from CITE-seq or the 10x multiome ATAC+RNA kit): 10xgenomics.com/event/integr…
Mike Kelly retweeted
My first thought when I saw this was - can you run this at home? And it turns out, you can!
biorxiv.org/content/10.1101/…
Mike Kelly retweeted
This is a great opportunity for someone interested in science communication and working to promote diversity in science. More info on the center here: multimodalintegration.org/
We are looking to hire a Director of Education and Outreach for @cegs_ica. If you'd like to help disseminate science and increase educational opportunities for students across NYC, please e-mail, DM, apply, or RT! More info at: nygenome-openhire.silkroad.c…
Mike Kelly retweeted
Excited to share our latest single cell multimodal assay. ASAP-seq pairs surface and intracellular protein detection with high quality chromatin accessibility on the @10xGenomics #scATACseq platform.
biorxiv.org/content/10.1101/…
Mike Kelly retweeted
A single-cell and single-nucleus RNA-Seq toolbox for fresh and frozen human tumors. dlvr.it/RfKpmC
Mike Kelly retweeted
So excited to share that my @NIGMS ESI MIRA (R35) was officially funded Friday! The grant (5 years) will support our efforts to develop better single-cell genomic technologies. If you're interested in working on single-cell tech in an affordable town, consider @UArizonaCMM @UofA!
Mike Kelly retweeted
We are very excited to present INs-seq, a new technology for massively parallel single-cell RNA-sequencing (scRNA-seq) integrated with recording of intracellular protein activities. Developed by @YonatanKatza @FadiSheban @AdamYalin and @AssafWeiner cell.com/cell/fulltext/S0092… (1/14)
Mike Kelly retweeted
Dear Immunologist,
Please consider applying as Phd/Postdoc/Researcher/Clinician scientist at my Single Cell Genomics Group @cnag_eu in #Barcelona. Lead the design and interpretation of #singlecell projects on #Immunotherapy #oncology and immune cell atlasing @humancellatlas.
Please RT:
WE ARE HIRING a (clinician) scientists specialized on IMMUNOLOGY for funded #singlecell projects in the @humancellatlas Immune Cell Atlas (B-cell lineage) and clinical trials on #immunotherapy #immunooncology.
BRIDGE THE GAP! APPLY HERE:
recruitment.crg.eu/content/j…