PhD in Molecular Biology interested in genomics, DNA repair and gene editing. Research Fellow at HMS/MGH. Former @IMBmainz and @EMBL

Boston, MA
Joined October 2020
Gabriel M. C. Longo retweeted
Today in @Nature we introduce INSTALL, which bypasses mammalian DNA immune sensing to enable non-viral DNA integration with recombinases—a step toward safe, scalable, and mutation-agnostic genome editing. 🧬 🧵(1/13) nature.com/articles/s41586-0… @BKleinstiver @CGM_MGH @MassGeneralNews @harvardmed
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Gabriel M. C. Longo retweeted
New Article! Multilevel characterization of genome editor nuclease activity with BreakTag bit.ly/4o0SFOD
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Gabriel M. C. Longo retweeted
.@SecKennedy - thank you so much for your broad support for gene editing and gene therapies as well as recognizing the importance of Baby KJ’s story and the potential that CRISPR base editing therapies hold for patients with severe genetic diseases. I’m thrilled you have publicly recognized the Herculean efforts that made KJs therapy possible, from the physicians @CHOP like @kiranmusunuru and Rebecca Ahrens-Nicklas to the technological innovation in @davidrliu and @BKleinstiver labs, and especially the coordination and cooperation of the regulators @US_FDA that made this possible. The gene editing medicine KJ received, a CRISPR adenine base editor was invented and developed in @davidrliu lab @Harvard with government grants from the NIH. This life-saving medicine, and so many future technologies, could not and will not be possible without government grants such as the one that supported this work, but are now shut off by this administration. So PLEASE ask this administration to stop attacking our academic institutions and restore government funding to @Harvard (where again, KJ’s medicine was created!!) We cannot have stories like KJ’s without full and unwavering support from the US government. Please help, please.
Thursday morning, I had the privilege of speaking with the Muldoon family, whose 10-month-old son, baby KJ, was discharged Wednesday from @ChildrensPhila. Baby KJ was diagnosed with a rare urea cycle disorder and received a first-of-its-kind personalized CRISPR gene-editing therapy—customized specifically for his unique mutation. Thanks to the extraordinary expertise and compassion of the team at CHOP and the researchers who developed this pioneering treatment, baby KJ now has a second chance at life. The Muldoon family’s courage is nothing short of inspiring, and I offer my deepest gratitude to everyone at CHOP and beyond who made this medical milestone possible. This isn’t just a medical success story—it’s a powerful glimpse into the future of personalized medicine. I remain firmly committed to working with @NIHDirector_Jay, @DrMakaryFDA, @DrOzCMS, and others to ensure that: • HHS continues to invest in cutting-edge research that drives breakthrough therapies • Our regulatory frameworks keep pace with rapid innovation • No family is ever denied access to life-saving treatments because of cost Breakthroughs like this are why we do the work.
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Gabriel M. C. Longo retweeted
Really nice characterization of Cas9 DSBs in human gDNA, with thorough off-target profiling and modeling of DSB outcomes for different guides. From @genomewizard @VassilisRozanas
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Gabriel M. C. Longo retweeted
In @nature we describe the use of scalable #proteinengineering & #machinelearning to predict millions of bespoke CRISPR-Cas9 enzymes, offering safer & more genome editing tools. 🧬🖥️ @RachelSilvers11 @CGM_MGH @MGH_RI @MassGeneralNews @harvardmed nature.com/articles/s41586-0…
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Gabriel M. C. Longo retweeted
A scientist who successfully treated her own breast cancer by injecting the tumour with lab-grown viruses has sparked discussion about the ethics of self-experimentation go.nature.com/3NXqzEq
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Gabriel M. C. Longo retweeted
Our Roukos lab discovered that cells use type II topoisomerases to regulate the 3D folding of DNA. This is important for controlling gene expression & may also be important for preventing DNA from becoming overtwisted & breaking. Findings published in Molecular Cell @cellpress.
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Gabriel M. C. Longo retweeted
The @RoukosVassilis lab led a new and detailed study of Topoisomerase II contribution to #3Dgenome folding in human cells and in different cell cycle phases -- my group and the @MundlosLab contributed analyses/data. The paper is now published in @MolecularCell ...1/n
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Gabriel M. C. Longo retweeted
Happy to share our new work in collaboration with the @MundlosLab @StefanMundlos & @AkisPapantonis labs, published today in @MolecularCell Many congratulations to all co-authors and many thanks to the funding agencies for the important support @imbmainz @sfb1361 @upatras
Online Now: Type II topoisomerases shape multi-scale 3D chromatin folding in regions of positive supercoils dlvr.it/TFwXmD
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Gabriel M. C. Longo retweeted
Online Now: Type II topoisomerases shape multi-scale 3D chromatin folding in regions of positive supercoils dlvr.it/TFwXmD
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Gabriel M. C. Longo retweeted
Join us as a Group Leader in #ComputationalBiology or #RNA Biology 🧬 More details via imb.de/jobs/scientific-posit… Application deadline is 5 January 2025
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Gabriel M. C. Longo retweeted
So happy to receive the GSCN Publication of the Year Award for our Stem Cell Zoo paper! 🐭🐰🐮🦏🐒 Congratulations to all co-authors.
Congratulation: @JorgeLazaroF & @EbisuyaMiki receive "GSCN 2024 Publication of the Year Award". All info here bit.ly/GSCNawards The award lecture: 26.9. in Jena at the GSCN Conference bit.ly/GSCNConference2024 @berlinnovation @PoLDresden @EMBLBarcelona #organoids #stemcells
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Gabriel M. C. Longo retweeted
Today in @NatureBiotech, we describe first-gen click editors (CEs), which couple the advantageous properties of DNA-dependent DNA polymerases and HUH endonucleases with RNA-programmable nickases for precise and versatile human genome editing. 🧬 nature.com/articles/s41587-0…
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Gabriel M. C. Longo retweeted
Click editing enables programmable genome writing using DNA polymerases and HUH endonucleases go.nature.com/3zPEIjp
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Gabriel M. C. Longo retweeted
Excited my PhD work is out now @NatureBiotech! We report Multiplexed Orthogonal Base Editor (MOBE) systems: tools to simultaneously install distinct point mutations. MOBEs introduce co-occurring genetic variants (polygenic disease, haplotypes)🧬 🧵⬇️ 1/ go.nature.com/3K8Kfn1 – at San Diego, CA
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Gabriel M. C. Longo retweeted
We are so proud of our colleague @genomewizard for publishing his amazing work🥹🥳 Congratulations to the first authors, @RoukosVassilis and all the team, for this great achievement 👏 ➡️Read the paper here: nature.com/articles/s41587-0…
It’s my pleasure to share that our latest manuscript is out now in Nature Biotechnology. This has been an incredibly exciting project! We describe BreakTag, an NGS-based tool for the rapid characterization of CRISPR nucleases and off-target activity. nature.com/articles/s41587-0…
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Gabriel M. C. Longo retweeted
Linking CRISPR–Cas9 double-strand break profiles to gene editing precision with BreakTag @NatureBiotech @RoukosVassilis @genomewizard nature.com/articles/s41587-0…
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